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Biosurfactants, green alternatives to potentially harmful synthetic surfactants, are structurally diverse molecules with polar and nonpolar regions. These natural molecules can reduce surface tension as a result of their amphipathic structures, allowing the measurement of surface tension to indicate changes in biosurfactant production. There is evidence that the presence of immiscible hydrocarbon results in increased biosurfactant production. This project aims to optimize biosurfactant production by marine bacterial isolates through alteration of culture conditions. Following the optimization of production, the biosurfactants will be extracted, purified, and structurally analyzed through thin-layer chromatography, infrared spectroscopy, and 1H nuclear magnetic resonance.
